Although you can't do it quite that directly, you can almost achieve it. Roll20 Audio Master is the script you want to use. As a Pro user, you have access to API scripts. If you've never used one, this video helps to explain how to install them: How to Install Roll20 API Scripts . Once you have installed Roll20AM, you can use its menu system to import a database from your jukebox playlist. This will allow you to make macros to play any of your audio. So while you can't click an icon on the desktop to play the sound, you can associate an icon with a token action button. You can select the door token for instance, and press a token action button to play the creaking sound. Or you could create a Chat Menu to make "soundboards" for specific dungeon levels.