Try disabling all your add-ons and clearing your cache again on Chrome. Go ahead and make sure you are giving permissions to Chrome to use your mic in Roll20. You can find the instructions for that in the Audio Visual Troubleshooting guide linked above by Steve. If you need help disabling your add-ons or clearing your cache, check out the instructions found here: Can you please follow Steps 1-3 of our Solving Technical Issues wiki page, taking special care to temporarily disable all browser add-ons and extensions, temporarily pause your anti-virus, and try switching browsers (for Chrome/Firefox specific issues) to see if one of those components is causing the problem. If all of that fails, please follow Step 4 , including providing all of the necessary information, so that we can further assist you.